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Ang mga tauhan sa Noli me tangere ay ang mga sumusunod:
1) Juan Crisostomo Ibarra
2) Maria Clara
3) Don Santiago delos Santos
4) Padre Damaso Vardolagas
5) Lieutenant Gueverra
6) Don Rafael Ibarra
7) Elias
8) Alfonso Linares de Espadana
9) Father Salvi 
10) Dona Pia
11) Basilio
12) Crispin
13) Sisa